Characterization of Rhizobia Isolated from Tigray Soil and Assessment of Their Effect on Germination and Seedling Vigor of Wheat and Field Pea
Table 8
Effect of rhizobia on wheat seed germination and 7-day-old seedling growth parameters.
Isolates
GP
SSL (cm)
SRL (cm)
SVI
SH1
97.0 ± 3.00def
4.96 ± 0.55lmo
6.52 ± 0.49hij
1113 ± 3.5o
SH2
99.0 ± 1.00abc
6.43 ± 0.61ghij
6.52 ± 0.57hij
1283 ± 2.0i
SH3
98.0 ± 0.00cd
4.79 ± 0.31mno
4.90 ± 0.44lmo
951 ± 1.5r
SH4
96.3 ± 1.15ef
6.26 ± 1.28ghijk
7.25 ± 0.62fgh
1301 ± 0.9i
SH5
95.8 ± 0.57f
5.86 ± 0.41hijkl
6.14 ± 0.52ijk
1148 ± 4.5mn
KO1
99.7 ± 0.57ab
11.60 ± 0.51a
8.57 ± 0.48de
2012 ± 1.6b
KO2
99.3 ± 0.57abc
7.85 ± 0.53de
9.28 ± 0.65cd
1702 ± 7.2f
KO3
100.0 ± 0.00a
6.06 ± 0.54ghijk
7.83 ± 0.55efg
1389 ± 9.5h
KO4
100.0 ± 0.00a
9.86 ± 0.64b
6.96 ± 0.71ghi
1683 ± 11.4f
KO5
99.3 ± 0.57abc
7.56 ± 0.52def
7.24 ± 0.64fgh
1471 ± 11.0g
KO6
99.0 ± 1.00abc
4.33 ± 0.39o
4.42 ± 0.52no
866 ± 22.2t
KO7
98.3 ± 1.52bcd
5.81 ± 0.58ijkl
5.73 ± 0.56jkl
1136 ± 10.3no
KO8
98.0 ± 1.00cd
5.90 ± 0.49hijk
5.14 ± 0.42lmo
1082 ± 17.1p
TS1
99.0 ± 1.00abc
5.32 ± 0.56klmn
5.46 ± 0.44klm
1069 ± 23.7p
TS2
98.0 ± 0.00cd
6.29 ± 0.53ghij
5.77 ± 0.41jkl
1183 ± 12.1kl
TS3
99.3 ± 0.57abc
5.84 ± 0.49hijkl
6.77 ± 0.55hi
1253 ± 43.9j
ME1
98.3 ± 0.57bcd
4.82 ± 0.54mno
5.25 ± 0.49klmn
991 ± 15.6q
ME2
99.7 ± 0.57ab
6.97 ± 0.58efg
10.13 ± 0.37c
1704 ± 6.7f
ME3
100.0 ± 0.00a
8.39 ± 0.23cd
13.90 ± 0.56a
2230 ± 12.1a
ME4
98.3 ± 0.57bcd
5.82 ± 0.34ijkl
6.42 ± 0.41hij
1204 ± 28.9k
ME5
97.3 ± 1.52de
5.55 ± 0.67jklm
6.14 ± 0.74ijk
1138 ± 45.3no
TA1
99.7 ± 0.57ab
8.04 ± 0.35cd
11.37 ± 0.61b
1935 ± 6.8d
TA2
99.7 ± 0.57ab
8.81 ± 0.18c
11.29 ± 0.65b
2004 ± 7.8bc
TA5
100.0 ± 0.00a
7.48 ± 0.74def
9.98 ± 0.52c
1747 ± 12.5e
TA7
99.0 ± 1.00abc
11.70 ± 0.71a
8.32 ± 0.33e
1983 ± 3.4c
TA8
99.7 ± 0.57ab
5.32 ± 0.65klmn
6.40 ± 0.68hij
1170 ± 10.3lm
TA9
99.0 ± 1.00abc
6.69 ± 0.38fghi
8.06 ± 0.67ef
1461 ± 10.5g
TA10
99.7 ± 0.57ab
6.76 ± 0.53fgh
8.11 ± 0.58ef
1483 ± 8.2g
NC
96.0 ± 0.00ef
4.71 ± 0.59mno
4.27 ± 0.63o
862 ± 2.6t
PC
99.3 ± 0.57abc
4.61 ± 0.56no
4.59 ± 0.62mno
915 ± 3.0s
LSD
1.65
0.94
0.92
26.25
SEm (±)
0.18
0.1
0.1
2.93
F-probability
<0.001
<0.001
<0.001
<0.001
CV (%)
1.02
8.6
7.75
1.16
Germination and shoot/root data are means of 3 replications and each replicate of the shoot/root length was an average of 10 wheat seedlings per petri-plates harvested after 7 days. Means in the same column that are followed by different letters are significantly different at using Fisher’s LSD test; LSD: least significant difference; CV: coefficient of variation (%); SEm: standard error of mean NC: negative control; PC: positive control; GP: germination percentage; SSL: seedling shoot length; SRL: seedling root length; SVI: seedling vigor index.